NucleoBond® Xtra Midi / Maxi

Catalog No. 740410, 740412, 740414, 740416

£1

For rapid small scale preparation high-purity plasmid DNA

Available: In stock

Product Description

Transfection-grade plasmid DNA, Blue LyseControl – efficient alkaline lysis

The new generation of anion exchanger – ultra-fast, transfection-grade plasmid DNA with high yield

• Optimized column design – Midi prep in approximately 30 min

• Column filter included – high filter flow rates, parallel lysate clearing, and loading onto the column

• Improved silica material – typical yields of 400 μg (Midi) and 1000 μg (Maxi)

• Proven anion-exchange technology – transfection-grade plasmid DNA

• NucleoBond® Xtra Plus kits contain NucleoBond® Finalizers to speed up DNA precipitation

• Anion-exchange chromatography technology

• Sample material: < 200 mL (high copy) and < 400 mL (low copy) E. coli culture

• Vector Size: < 300 kbp

• Ratio A260/A280: 1.80–1.85

NucleoBond® Kits

  • Kit contents :
    • NucleoBond® Xtra Midi /Maxi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A

NucleoBond® Xtra Midi kits

  • Reference numbers :
    • 10 preps: 740410.10
    • 50 preps: 740410.50
    • 100 preps: 740410.100

NucleoBond® Xtra Maxi

  • Reference numbers :
    • 10 preps: 740414.10
    • 50 preps: 740414.50
    • 100 preps: 740414.100

NucleoBond® Plus Kits

  • Kit contents :
    • NucleoBond® Xtra Midi / Maxi Columns with inserted NucleoBond® Xtra Column , Filters, NucleoBond® Finalizers, buffers, RNase A

NucleoBond® Xtra Midi Plus

  • Reference numbers :
    • 10 preps: 740412.10
    • 50 preps: 740412.50

NucleoBond® Xtra Maxi Plus

  • Reference numbers :
    • 10 preps: 740416.10
    • 50 preps: 740416.50

Product accessories

  • NucleoBond® Xtra Buffer Set I, for isolation of low-copy plasmids, cosmids, BACs, PACs, and P1 constructs, only applicable with NucleoBond® Xtra kits, sufficient for 10 Xtra Maxi preps or 20 Xtra Midi preps. Set includes 150 mL buffers RES, LYS, NEU, and RNase A, 740417
  • High-copy plasmids from E. coli
  • Low-copy plasmids from E. coli (increased buffer volumes required, see “Ordering”)

MACHEREY-NAGEL has developed NucleoBond® Xtra, a new generation of anion exchangers based on the patented NucleoBond® technology. With NucleoBond® Xtra typical yields of ≥ 250 μg (Midi) or ≥ 1000 μg (Maxi) of ultra-pure plasmid DNA can be obtained in about half of the time compared to other Midi and Maxi kits based on anion-exchange chromatography.
NucleoBond® Xtra Midi and Maxi kits contain enlarged columns which leads to lower silica resin beds. This in turn enables faster flow of lysate and buffers through the columns. Specially designed column filters are included for convenient and time-saving clarification of bacterial lysates. The column filters are supplied inserted in the NucleoBond® Xtra columns and allow parallel clarification of bacterial lysate and loading onto the column. Their large, structured surface leads to high filter flow rates and minimized risk of clogging. The improved silica material with greater DNA binding capacity is based on the proven NucleoBond® anion-exchanger group MAE, methylaminoethanol (patented technology). Optimized buffer compositions additionally lead to improved alkaline lysis and increased column flow rates.

Bacteria are harvested from an overnight culture and lysed by an optimized alkaline lysis procedure. The bacterial lysate is cleared and loaded onto the equilibrated column in one step (plasmid DNA binds to the anion-exchange resin). The first washing step using equilibration buffer is performed with inserted column filter to wash out residual lysate from the filter and obtain maximum recovery of DNA. After subsequent washing, the purified plasmid DNA is eluted in a high-salt buffer and precipitated with isopropanol. Precipitation can be performed by centrifugation (NucleoBond® Xtra Midi or Maxi) or by using the NucleoBond® Finalizer / Finalizer Large included in the NucleoBond® Xtra Midi Plus/Maxi Plus kits to reduce the total prep time to about 30 minutes (Midi Plus) or 35 minutes (Maxi Plus).

  • PLoS One. 2008;3(11):e3647. doi: 10.1371/journal.pone.0003647. Epub 2008 Nov 5.
    A one pot, one step, precision cloning method with high throughput capability.
    Engler C, Kandzia R, Marillonnet S.
    View Abstract

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