Post clean-up and concentration of large sized DNA
• Effective post clean-up and concentration of large DNA fragments for successful downstream
• High recovery rates up to 90 % for DNA fragments 100 bp–50 kbp
• Concentrate your DNA sample easily and faster compared to microdialysis filtrations units
• 15 min/10 preps – time-saving procedure
• Silica-membrane technology
- Kit contents :
- NucleoSpin® gDNA Clean-up Columns (light green rings), Collection Tubes (2 mL), Binding Buffer DB, Wash Buffer DW, Elution Buffer DE)
- Reference numbers :
- 10 preps: 740230.10
- 50 preps: 740230.50
- 250 preps: 740230.250
- Clean-up and concentration of pre-purified DNA (organic extractions)
- Clean-up and concentration of DNA from enzymatic reactions
- Not recommended for gel or PCR clean-up! For this application, please use NucleoSpin® Gel and PCR Clean-up.
- Typical downstream applications: qPCR, enzymatic reactions, Southern blotting, STR amplification
The NucleoSpin® gDNA Clean-up procedure is the easiest and fastest way to clean up large-sized DNA from enzymatic reactions, standard “organic” extraction methods or for buffer exchange. Thus, this kit can be used to remove contaminants such as salts, enzymes, nucleotides, labeling reagents, etc. from a DNA sample.
The kit provides a simple and convenient way to purify DNA by selective binding to a silica membrane. The proven silica membrane technology is an effective alternative to the widely used concentrator columns for cleaning and concentrating samples, which were extracted by standard “organic” methods. The procedure follows a simple bind-wash-and-elute procedure (see figure).
In the NucleoSpin® gDNA Clean-up procedure DNA binds to the silica membrane in the presence of a high concentration of chaotropic salt, using Buffer DB. Contaminations are removed in a double wash step with ethanolic buffer. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline buffer DE (5 mM Tris/HCl, pH 8.5) or water.
- Chemosphere. 2013 May;91(9):1338-43. doi: 10.1016/j.chemosphere.2013.01.114. Epub 2013 Mar 5.
Real-time PCR for rapidly detecting aniline-degrading bacteria in activated sludge.
Kayashima T1, Suzuki H, Maeda T, Ogawa HI. Choi PS, Meyerson M.