NucleoSpin® RNA Virus

Catalog No. 740956

£1

For reliable purification of viral RNA and DNA from cell-free biological fluids

Available: In stock

Product Description

Viral RNA and DNA from serum and plasma
For reliable purification of viral RNA and DNA from cell-free biological fluids

• Reliable and reproducible isolation of viral RNA and DNA* – free of impurities

• Safe and well-established procedure

• Proven for a large variety of viruses including influenza viruses, HCV, HIV, Bird Flu Virus, Blue Tongue Virus

• Carrier RNA included for highest sensitivity in downstream applications

• Fast and convenient protocol – viral RNA and DNA* within only 30 min

• Silica-membrane technology

• Sample material: < 150 µL serum, plasma, cell-free biological fluids

• Fragment size: 100 bp–approx. 50 kbp

  • Kit contents :
    • NucleoSpin® RNA Virus Columns with Collection Tubes, Collection Tubes (2 mL), buffers, Carrier RNA
  • Reference numbers :
    • 10 preps: 740956.10
    • 50 preps: 740956.50
    • 250 preps: 740956.250
  • Product accessories :
    • Kit for use on QIAcube®, NucleoSpin® RNA Virus Columns, buffers, Carrier RNA, 740956.240C
      Proteinase K, 100 mg, 740506
  • Viral RNA from 150 μL serum, plasma, cell-free biological fluids
  • HCV, HBV, HAV, HIV, HSV, HPV, VZV, EBV, parvovirus B19, H5N1, H1N1
  • Support protocol for parallel isolation of viral RNA and DNA
  • Typical downstream applications: RT-PCR, enzymatic reactions
  • For parallel isolation of viral RNA and DNA use of Proteinase K required (not included in the kit)

NucleoSpin® RNA Virus is designed for the isolation of viral nucleic acids from serum, plasma, or any cell-free biological fluids. NucleoSpin® RNA Virus features a special type of membrane which has a high binding capacity for nucleic acids. The procedure is rapid and easy. The lysate is incubated for 5–10 min and applied to the NucleoSpin®column. After two washing steps to remove contaminations the RNA can be eluted with water or low-salt buffer. The kit contains all necessary buffers, spin columns and detailed protocols.
Generally, NucleoSpin® RNA Virus is suited for the simultaneous purification of viral RNA and DNA (support protocol). For example, isolation of HBV DNA is performed with an additional Proteinase K digest (enzyme not included in the kit). For isolation of viral DNA with highest sensitivities, the NucleoSpin® Blood kit is recommended.

  • Sci Rep. 2016 Nov 3;6:35962. doi: 10.1038/srep35962.0-x
    Improving virus production through quasispecies genomic selection and molecular breeding.
    Pérez-Rodríguez FJ, D’Andrea L, de Castellarnau M et al
    View Abstract