Sepadextran™ is a beaded gel filtration medium prepared by crosslinking dextran and it is supplied in its dry form. Sepadextran™-25 can be used for protein and nucleic acid purifications being the exclusion limit 5 kD for proteins and 10 bases for nucleic acids. Desalting (before IEX and after HIC or Affinity Chromatography) & buffer exchange (between different chromatography steps) are other uses.
Fine grade are for preparative separations and routine laboratory work.
- Kit contents:
- X ml of Sephadextran25fine resin as a bulk resin.
- Cat. numbers:
- 100ml resin: SD25F-100
- 500ml resin: SD25F-500
- Product Name : Sepadextran™-25 Fine
- Cat. No. : SD25F-X
- Matrix : Crosslinked dextran
- Water Regain : 2.15-2.25 ml/g
- Swelling : 4-6 ml/g
- Dry Particle Size : 20-80 µm (>80%)
- Wet Particle Size : 35-140 µm
- Maximum Operating Pressure : Generally obeys Darcy’s Law: U=Ko ΔP/L Where: U= linear flow rate(cm/h). ΔP= pressure drop over gel bead (cm H2O). L= bed high(cm). Ko=30
- Fractionation Range : 1.5 kD for globular proteins, 100-5,000 D for dextrans
- pH Stability : 2.0 to 13
- Autoclavable : 121˚C, pH 7.0 (30 minutes)
- Chemical Stability : All commonly used buffers, including: 0.1 M NaOH; 0.01 M HCl; 1 M acetic acid; 8 M urea; 6 M guanidine HCl; 1% SDS, 24% Ethanol; 30% Propanol; 30% Acetonitrile
- Storage Temperature : Ambient
- No article
INSTITUT FÜR BIOLOGIE BERLIN, HUMBOLDT- UNIVERSITÄT ZU BERLIN, GERMANY
“We are studying the function and mechanisms of cofactor-containing enzymes. The focus is on the function of complex metal clusters and flavins in redox catalysis. Therefore we need a lot of purified proteins from various organisms. The empty FPLC Columns from ABT are very helpful for our working with different chromatographic material. The handling is good and also the quality and price.”
INSTITUTE OF BIOTECHNOLOGY, UNIVERSITY OF MANCHESTER, UK
“The advantage of using ABTs Zn2+ resin in routine protein purifications is the requirement for fewer wash steps, providing the possibility for significant time and cost savings” In the purification of a protein, the performance of the resin in terms of duration of the overall process/number of steps required is just as important as the purity of the resulting protein. For clients using our products on a larger scale, the number of process steps therefore becomes an important economic consideration”.
UNIVERSITY OF GRONINGEN, DEPARTMENT OF CHEMICAL ENGINEERING, NETHERLANDS
“Agarose Bead Technologies facilitates this screening by offering their range in immobilization beads in a convenient test kit. This makes selection of the best carrier possible without the need to buy relatively large (and expensive) amounts of resins.” Sometimes the only way to know which purification or immobilization resin is the best for each work is to test with different degrees of activations. ABT offers inexpensive test kits for prior practical evaluation of the resin that will give best results in your process.
CENTRO DE INVESTIGACIONES BIOLÓGICAS-CSIC, DEPARTMENT OF MOLECULAR MICROBIOLOGY, MADRID, SPAIN
“Several companies offer Nickel activated chelating beads; however, Agarose Bead Technologies (ABT) has taken a different approach and offers a comprehensive range of beads with different chelating metals (nickel, cobalt, copper, zinc) so that a much wider range of proteins can be effectively separated. In addition, the chelates are offered at different loading capacities (Low, High, Very High) so that the beads can deal effectively with technical issues such as different sizes of proteins, recovery yields and specificity. ABT offers test kits with a range of chelates in small quantities so that you can optimize your separation for a very low price. The chelates have been very widely accepted by research labs and we have several testimonials available”.
RENSSELAER POLYTECNNIC INSTITUTE, DEPARMENT OF CHEMICAL AND BIOLOGICAL ENGINEERING, USA
“In our sulfotransferase immobilization experiments, enzyme immobilized on ABT’s Glyoxal Agarose beads showed highest activity and stability in comparing with other beads.” Research projects in biochemical engineering emphasize biocatalysis, bioseparations,and metabolic engineering. Fundamental and applied aspects of enzyme technology,mammalian cell culture, membrane sorption and separation, displacement chromatography,and salt-induced precipitation are important areas of focus.
INSTITUTE OF CATALYSIS AND PETROCHEMISTRY, INTERNATIONAL EXCELLENCE CAMPUS UAM+CSIC MADRID, SPAIN
“One of our recent projects is the synthesis of prebiotic oligosaccharides. Prebiotics are non-digestible food ingredients that are selectively fermented by the human gastrointestinal microbiota allowing specific changes, both in its composition and/or activity, and conferring benefits upon host well-being and health. Prebiotics emulate human milk oligosaccharides,a family of hundreds of structurally diverse carbohydrates that provide benefitsto breast-fed infants. We are investigating the synthesis of galactooligosaccharides (GOS) and fructooligosaccharides (FOS) using glycosidic enzymes, in particular ßgalactosidasesand ß-fructofuranosidases,respectively. In addition, immobilization protects enzymes from inactivation at extreme pH, high temperatures and organic solvents”.
DEPARTMENT OF MOLECULAR MICROBIOLOGY·CENTRO DE INVESTIGACIONES BIOLÓGICAS (CIB-CSIC) MADRID, SPAIN
In my lab at CIB-CSIC, we have been using (since 1996) Ni2+-IMAC for the purification of many different His-tagged proteins at large scales (10-100 mg). We are very demanding in terms of the yields and the final purity grade of our protein preps, because both of them are key factors in the structural and biochemical characterization of proteins, our main research interest. One of the major recent improvements in our daily work has been the discovery of the high-density IDA-agarose beads developed and manufactured by ABT: They match (and in some occasions, even outperform) the results previously achieved with other commercial metal-chelating supports, specially when packing of relatively large volumes (> 5 ml) of gel are required and there is a need for a mechanically robust and reliable, yet affordable, chromatographic material. Thus ABT beads have been included as essential resources in all our recent scientific achievements.
UNIVERSITY OF CAMBRIDGE DEPT OF CHEMISTRY, UK
“We have since purchased 500 ml of Agarose Bead Technologies resin and have used this to purify several proteins in our lab. We have purified all-alpha helical and all-beta sheet proteins, from 100 – 300 amino acids in size, expressed in E.coli. We find that the ABT resin performs as well as that of other leading manufacturers but costs less. This has allowed us to make a significant saving as we work on many mutant proteins and therefore use a lot of pre-charged resin”.
DEPARTMENT OF MICRO AND NANOTECHNOLOGY TECHNICAL UNIVERSITY OF DENMARK, DENMARK
“In our research within micro-biotechnology we often have a need for customized solutions that suits our technology platform. Agarose Bead Technologies has provided us with excellent customized beads in a short time, which was essential for the successfulness of our research project. Thus, we have no problems recommending ABT when it comes to product quality and delivery.”
DEPARTMENT OF REGULATION BIOCHEMISTRY, KITASATO UNIVERSITY GRADUATE SCHOOL OF MEDICAL SCIENCES, SAGAMIHARA, KANAGAWA, JAPAN
“We have been studying about the patho-physiological alteration of the mucins in gastrointestinal mucosa (1) and we use (since 2004) ABT-agarose beads (Plain 8% Agarose Beads Standard) column (1.0×50cm) for the analysis of mucin. The void volume fraction (Fr-1) eluted with the Triton-Tris buffer can be collected as mucin. Agarose Bead Technologies resins are easy to use and reliable substance to isolate the complex macromolecules, and reasonable because they are washable and reusable. So we can recommend ABT for separating high molecular glycoconjugates”. (1) Azuumi Y et al. (1980) Gut 21:533-6
ZMBH ZENTRUM FÜR MOLEKULARE BIOLOGIE DER UNIVERSITÄT HEIDELBERG, GERMANY
“My group is regularly expressing and purifying recombinant proteins, mostly in E.coli, which we routinously purify using Metal Affinity Chromatography. For a couple of years, we are now using Nickel-agarose support purchased from Agarose Beads Technology (ABT). In the applications we are using these beads (gravity flow standard purification of His-tagged proteins), the product is working reproducibly well and reliable and we have now switched to ABT beads for all our purifications”.
SANDIA NATIONAL LAB LIVERMORE, CA USA
“I have used the Low Density Glyoxal 4 Rapid Run Fine Agarose beads, Streptavidin Rapid Run Fine Agarose beads, and the new Low Density Glyoxal 4 Highly Cross-Linked Superfine 17 um Agarose beads. They all have high level of affinity binding and low level of non-specific background, offering consistent and reliable performance for my applications. The 17 um beads can be packed more densely and uniformly and can be