SINEUP

Catalog No. GE01

£1

A modular range of tools for analysis and detection of exosomes

Available: In stock

Product Description

SINEUPTM non-coding RNA technology represents a breakthrough for the study and manipulation of eukaryotic genes. SINEUPTM allows up to 10-fold increases in expression levels of target proteins by increasing the efficiency of translation. It has been proposed that the mechanism is similar to IRES elements which increase the efficiency of translation initiation. Transcription of the target gene is unaltered. Similar to RNAi, which can be used to knockdown (down-regulate) protein levels, SINEUPTM technology does not rely on the direct manipulation of the target gene.

SINEUP Effector Domain

  • GE01, pUC19-ED SINEUP effector domain, 2ug

Use SINEUPTM to

  • Increase bio-manufacturing productivity
  • Increase in-vitro translation yields
  • Validate microarray experiments
  • Manipulate cell phenotype
  • Screen new drug targets
  • Screen a gene across different cell lines
  • Screen genetic variants

The cellular machinery that generates up-regulation of protein expression with SINEUPTM constructs has been shown to work for multiple genes and to be present in a range of mammalian cell types including murine and human cells. SINEUPTM was developed following identification and characterization of the long non-coding RNA (lncRNA) AS Uchl1 in neuronal cells.
The ability of SINEUPTM constructs to increase protein expression is not restricted to Uchl1.  In HEK293 cells a SINEUPTM construct incorporating an antisense motif for GFP increases levels of GFP protein without any increase in GFP mRNA levels
SINEUPTM can be combined with other genetic tools that act at the mRNA level to increase the overall amount of protein expression that can be achieved from a given cell type.
SINEUPTM non-coding RNA technology represents a breakthrough for the study and manipulation of eukaryotic genes. SINEUPTM allows up to 10-fold increases in expression levels of target proteins by increasing the efficiency of translation. It has been proposed that the mechanism is similar to IRES elements which increase the efficiency of translation initiation. Transcription of the target gene is unaltered. Similar to RNAi, which can be used to knockdown (down-regulate) protein levels, SINEUPTM technology does not rely on the direct manipulation of the target gene.
Characterization of the SINEUPTM system, published in Nature (and unpublished data) have demonstrated increases in protein levels as high as 10-fold. mRNA levels are unaffected. SINEUPTM technology provides an additional layer of control over protein production which can be combined with existing tools.

  • Gene. 2015 Sep 15;569(2):287-93. doi: 10.1016/j.gene.2015.05.070. Epub 2015 Jun 2.
    Engineering mammalian cell factories with SINEUP noncoding RNAs to improve translation of secreted proteins.
    Patrucco L, Chiesa A, Soluri MF et al.
    View Abstract
  • RNA Biol. 2015;12(8):771-9. doi: 10.1080/15476286.2015.1060395.
    SINEUPs: A new class of natural and synthetic antisense long non-coding RNAs that activate translation.
    Zucchelli S, Cotella D, Takahashi H et al.
    View Abstract
  • Front Cell Neurosci. 2015 May 13;9:174. doi: 10.3389/fncel.2015.00174. eCollection 2015.
    SINEUPs are modular antisense long non-coding RNAs that increase synthesis of target proteins in cells.
    Zucchelli S, Fasolo F, Russo R et al.
    View Abstract

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