NucleoBond Xtra Midi kit

NucleoBond Xtra Midi kit for transfection-grade plasmid DNA

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  • Product Name: NucleoBond Xtra Midi kit for transfection-grade pl...
  • Cat. No.: 740410
  • Package unit: 50, 250 Reactions
  • Company: Macherey-Nagel
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2nd generation of anion exchanger for fast purification of plasmid DNA


NucleoBond Xtra Midi kit for transfection-grade plasmid DNA

2nd generation of anion exchanger for fast purification of plasmid DNA

Application
Transfection-grade plasmid DNA isolation
Target
Plasmid DNA
CE certified
No, research use only
Technology
Anion exchange chromatography
Brand
NucleoBond
Format
Midi prep
Package unit
10 Prep(s), 50 Prep(s), 100 Prep(s)
Handling
Gravity flow
Lysate clarification
Column filter
Automated use
No
Sample material
Bacteria, E. coli
Sample amount
< 200 mL (high copy), < 400 mL (low copy)
Vector size
< 300 kbp
Typical yield
500 µg
Theoretical binding capacity
800 µg
Typical purity A260/A280
1.8−1.95
Endotoxin level
1−10 EU/µg DNA
Isopropanol precipitation
Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi
Preparation time
70 min/prep
Typical downstream application
Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature
18−25 °C
Shelf life (from production)
39 Months
Hazardous material
Yes
Product Name
NucleoBond Xtra Midi kit for transfection-grade plasmid DNA
Cat. No.
740410
Package unit
50, 250 Reactions
Company
Macherey-Nagel
Origin
Germany
Storage Temperature
4−30 °C
Shelf life (from production)
39 Months
Typical downstream application
Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells