2nd generation of anion exchanger for fast purification of plasmid DNA
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NucleoBond Xtra Midi kit for transfection-grade plasmid DNA
2nd generation of anion exchanger for fast purification of plasmid DNA
| Application | Transfection-grade plasmid DNA isolation |
| Target | Plasmid DNA |
| CE certified | No, research use only |
| Technology | Anion exchange chromatography |
| Brand | NucleoBond |
| Format | Midi prep |
| Package unit | 10 Prep(s), 50 Prep(s), 100 Prep(s) |
| Handling | Gravity flow |
| Lysate clarification | Column filter |
| Automated use | No |
| Sample material | Bacteria, E. coli |
| Sample amount | < 200 mL (high copy), < 400 mL (low copy) |
| Vector size | < 300 kbp |
| Typical yield | 500 µg |
| Theoretical binding capacity | 800 µg |
| Typical purity A260/A280 | 1.8−1.95 |
| Endotoxin level | 1−10 EU/µg DNA |
| Isopropanol precipitation | Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Midi, NucleoSpin Finisher Midi |
| Preparation time | 70 min/prep |
| Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
| Storage temperature | 18−25 °C |
| Shelf life (from production) | 39 Months |
| Hazardous material | Yes |
Product Name
NucleoBond Xtra Midi kit for transfection-grade plasmid DNA
Cat. No.
740410
Package unit
50, 250 Reactions
Company
Macherey-Nagel
Origin
Germany
Storage Temperature
4−30 °C
Shelf life (from production)
39 Months
Typical downstream application
Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells