DEAE Rapid Run™ Agarose Resin is a highly crosslinked matrix with a bead size of 50-150 µm activated with diethylaminoethyl. It is developed to perform Ion Exchange Chromatography and separate biomolocules on the basis on their net surface charge. ABT offers two different types of Anion Exchangers depending on their interaction strength: Q Rapid Run™ as a Strong Anion Exchanger and DEAE Rapid Run™ as a Weak Anion Exchanger.
Related
Deneka, Dawid et al. “Structure of a Volume-Regulated Anion Channel of the LRRC8 Family.” Nature 558.7709 (2018): 254–259. Nature.
Petrenčáková, Martina et al. “Conformational Properties of LOV2 Domain and Its C450A Variant within Broad PH Region.” Biophysical Chemistry 259 (2020): n. pag. Biophysical Chemistry.
Qian, Yong et al. “A Genetically Encoded Near-Infrared Fluorescent Calcium Ion Indicator.” Nature Methods 16.2 (2019): 171–174. Nature Methods.
Dunne, Matthew, and Martin J. Loessner. “Modified Bacteriophage Tail Fiber Proteins for Labeling, Immobilization, Capture, and Detection of Bacteria.” Methods in Molecular Biology. Vol. 1918. Humana Press Inc., 2019. 67–86. Methods in Molecular Biology.
Huo, Jiangdong et al. “Structural Characterisation of a Nanobody Derived from a Naïve Library That Neutralises SARS-CoV-2.” (2020): n. pag. Web.
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DEAE Rapid Run™ Agarose Resin is a highly crosslinked matrix with a bead size of 50-150 µm activated with diethylaminoethyl. It is developed to perform Ion Exchange Chromatography and separate biomolocules on the basis on their net surface charge. ABT offers two different types of Anion Exchangers depending on their interaction strength: Q Rapid Run™ as a Strong Anion Exchanger and DEAE Rapid Run™ as a Weak Anion Exchanger.
| Product Name | DEAE Rapid Run™ Agarose Bead |
| Cat. No. | DEAERR-X |
| Bead Geometry & Size | Spherical ~50 - 150 µm |
| Bead Mean Diameter d50v | 90 µm |
| Crosslinked | Highly Crosslinked |
| Exclusion Limit Da | > 4x10^6 |
| pH Stability Working Range | 3 - 14 |
| Working Temperature | 4 - 30 ºC |
| Chemical Stability | Most commonly used aqueous and organic solutions including: 1 M NaOH, 8 M urea, 30% isopropanol and 70% ethanol |
| Ligand | Diethylaminoethyl |
| Ion Exchanger Type | Weak Anion Exchanger |
| Flow Velocity (cm/h) at 3 bar in 1,6 x 15 cm column | > 900 |
| Binding Capacity | > 50 mg BSA/mL resin |
| Ionic Capacity (mmol Cl- /ml resin) | 0.10 - 0.15 |
| Static Binding Capacity | > 60 mg BSA/ml resin |
| Antimicrobial Agent | 20% ethanol |
| Storage Temperature | 4 - 30˚C |